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  Program
   



 

   

Tuesday, November 01, 2011

 




Location: Mission City Ballroom


8:00 - 9:00

Registration & Breakfast



9:00 - 9:10

Opening Remarks


9:10 - 10:00 - Keynote

Semiconductor Sequencing for life: Democratizing Sequencing
Jason Myers, Ph.D., Group Leader, Ion Torrent Systems, Inc., CA, USA


10:00 - 10:30

An analysis of the reliability of qPCR data published in the peer-reviewed literature
Stephen A. Bustin, BA(Mod) Ph.D. (TCD), Professor of Molecular Science, Blizard Institute of Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, Queen Mary University of London,UK


10:30 - 11:00

MIQE challenges DNA and RNA quantification on the single cell-level
Michael Pfaffl, Ph.D., Professor of Molecular Physiology, Technical University Munich, Germany


11:00 - 11:20

Coffee break


11:20 - 11:50

Immune Repertoire Profiling by High-Throughput Sequencing
Jiankui He, Ph.D., Stanford University, CA, USA


11:50 - 12:20

RNA-seq profiling immune subpopulations to enable drug target discovery
Achim K. Moesta, Ph.D., Scientist, Genome Analysis Unit, Amgen Inc., USA


12:20 - 13:15

Lunch


13:15 - 13:45

Taking a deep look into Biology with Single Cell Expression Profiling
Mikael Kubista, Ph.D., Professor, Institute of Molecular Genetics, Prague, Czech Republic and Chairman, TATAA Biocenter, Sweden



13:45 - 14:15

non-coding RNA RT-qPCR gene expression profiling and dissection of LncRNA function through integrative genomics
Dr. Jo Vandesompele, Professor in functional cancer genomics and applied bio-informatics, Ghent University and CEO, Biogazelle, Belgium


14:15 - 14:45

Accurate, High-Throughput, Quantitative Gene Expression for Biomarker Profiling and Validation
David Ginzinger, Ph.D., VP of Genomics Research and Applications, WaferGen Biosystems, Inc. CA, USA


14:45 - 15:15

Principles of Optimal PCR Design and Application to the 2009 H1N1 Pandemic Influenza Virus
John SantaLucia, Ph.D., CEO, DNA Software, Inc., USA


15:15 - 16:00

Poster presentation and coffee break


16:00 - 16:30

qPCR solutions and minimizing variation in the workflow
Ian Kavanagh, Ph.D., Senior R&D Manager, Thermo Fisher Scientific, Finland


16:30 - 17:00

A new SYBR Green-based qPCR system that does not generate primer dimers - Its applications and enzymatic mechanism
Larry Xi, Ph.D., Senior Staff Scientist, Life Technologies Corporation, CA, USA


17:00 - 18:00

Ways to integrate qPCR into the NGS Workflow - library validation and validation of results - Panel Discussion

Moderator:
Kevin L. Knudtson, Ph.D., Director, DNA Facility, University of Iowa, IA, USA

Panel members:
Jason Myers, Ph.D., Group Leader, Ion Torrent Systems, Inc., CA, USA
Gregory L. Shipley, Ph.D., Asst. Professor, Director, Quantitative Genomics Core Laboratory, University of Texas Health Science Center- Houston, TX, USA
Deborah S. Grove, Ph.D., Director, Genomics Core Facility, Huck Institute for Life Sciences, Penn Sate University, PA, USA


18:00 - 19:00

Reception



   

Wednesday, November 02, 2011

 





Location: Mission City Ballroom


8:00 - 9:00

Registration & Breakfast



9:00 - 9:10

Opening Remarks


9:10 - 10:00 - Keynote

Integrated genomic technologies for biological studies
Mostafa Ronaghi, Ph.D., SVP & CTO, Illumina Inc., CA, USA


10:00 - 10:30

Using Whole Cell Lysates for mRNA and miRNA Detection
Gregory L. Shipley, Ph.D., Asst. Professor, Director, Quantitative Genomics Core Laboratory, University of Texas Health Science Center- Houston, TX, USA


10:30 - 11:00

Direct Detection of Circulating Tumer Cells in Blood by Digital CastPCR
David Deng, Ph.D., Sr. Manager, Genomic Assay R&D, Life Technologies Corporation, USA


11:00 - 11:20

Coffee break


11:20 - 11:50

A novel approach for simultaneous detection of the most common food-borne pathogens by multiplex qPCR
Emir Hozdic, D.V.M., Ph.D., Director, Center for Comparative Medicine, Real-time PCR Research & Diagnostics Core Facility, University of California, Davis, CA, USA


11:50 - 12:20

Real Time Ligase Chain Reaction with Thermo-Sensitive Acceptor Probes: Application to SNP Detection
Alexandre Lebedev, Senior Principal Scientist, TriLink BioTechnologies, Inc., CA, USA


12:20 - 13:15

Lunch


13:15 - 13:45

Short Amplicon PCR Applied to Casework Samples
Valorie Ryan, Ph.D., Associate Section Manager, MRI Global, FL, USA
Jason D.Bannon, Ph.D., Senior Biological Scientist, Program Advisor, FBI Laboratory Division, VA, USA


13:45 - 14:15

Applications of qPCR in Agricultural Biotechnology
Sandra Meyer, Senior Research Associate, Pioneer Hi-Bred, International Inc., a DuPont Company, IA, USA


14:15 - 14:45

Implementing Next-Generation Sequencing in a Core Facility
Deborah S. Grove, Ph.D., Director, Genomics Core Facility, Huck Institute for Life Sciences, Penn Sate University, PA, USA


14:45 - 15:30

Poster presentation and coffee break


15:30 - 16:00

Rare sequence variant detection using NGS in a core setting
Kevin L. Knudtson, Ph.D., Director, DNA Facility, University of Iowa, IA, USA


16:00 - 16:30

A Systematic Comparison and Evaluation Of High Density Exon Arrays and Next Generation Sequencing Technology in Unraveling the Transcriptome of Sickle Cell Disease
Nalini Raghavachari, Ph.D., Director, NHLBI Genomics Core, National Institute of Health, USA


16:30 - 17:00

Automated sample preperation platform for Next Generation DNA sequencing using a digital microfluidic hub
Hanyoup Kim, Ph.D., Postdoctoral research scientist, Biosystems Research Group, Sandia National Laboratories, USA


17:00 - 17:15

End of Meeting Comments


Note: Program time and speakers may change


We are unable to announce our regular workshops with fee for this year due to unavoidable circumstances. We are open to host free workshop/s on Nov. 03 if possible.



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