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Hosted By
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Monday, 29th, October 07
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8.00 - 9.00
Registration & Breakfast
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9.00 - 9.10
Opening Remarks
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9.10 - 9.50
Keynote - Real-time PCR in Research and Diagnostics
Speaker:
Russell Higuchi, Ph.D., Associate Director, Human Genetics Dept., Roche Molecular
Systems, CA, USA
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9.55 - 10.20
Quantitative Immuno-PCR for Infectious Diseases
Speaker:
Niel T. Constantine, Ph.D. Professor of Pathology, Director- Clinical Immunology,
University of Maryland School of Medicine, Baltimore, MD, USA
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10.20 - 10.40
Cofee Break
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10. 40 - 11.05
Molecular staging of colorectal cancer
Speaker:
Stephen A. Bustin, Ph.D. FRSM, Professor of Molecular Science, Queen Mary's
School of Medicine and Dentistry, University of London, UK
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11.05 - 11.30
Selection of Prognostic Biomarkers by Quantitative RT-PCR Analysis
Speaker:
Roberto A. Macina, Ph.D., Director Molecular Technology, diaDexus, Inc.,
CA, USA
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11.30 - 11.55
Using Gene Expression qPCR to Identify Diagnostic Biomarkers
Speaker:
Maureen Cronin, Ph.D., VP Technology Research, Genomic Health Inc., CA, USA
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11.55 - 12.20
High-throughput PCR Techniques for Profiling RNA and DNA from Archived Tumor Tissue
Speaker:
Philip Bernard, MD, Huntsman Cancer Center, University of Utah, USA
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12.20 - 13.20
Lunch Break
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13.20 - 13.45
Proximity Ligation Assay: Using qPCR for the Quantification of Proteins
Speaker:
David W. Ruff, Ph.D., Senior Staff Scientist, Proximity Ligation Assay R&D
Team Leader, Applied Biosystems, CA, USA
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13.45 - 14.10
Multi-Discipline Analysis of Gene Silencing: Complementary use of Multiplex RT-qPCR,
2-D electrophoresis and Western blotting for RNAi based pathway analysis
Speaker:
Eli Hefner, Ph.D., Senior Scientist, Bio-Rad Laboratories, CA, USA
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14.10 -14.35
Providing Real-Time PCR Technology in a Core Facility
Speaker:
Deborah S. Grove, Ph.D., Director of Research Projects, Nucleic Acid Facility,
Huck Institutes of the Life Science, The Pennsylvania State University, PA, USA
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14.35 - 15.00
Real-time PCR expression profiling
Speaker:
Mikael Kubista, Ph.D., Founder, TATAA Biocenter, Sweden
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15.00 - 16.15
Poster Session and Coffee Break
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16.15 - 16.40
Small RNAs in Total RNA: understanding miRNA preparations
Speaker:
Marc Valer, Solutions Manager - Life Sciences, Agilent Technologies, Inc.,
CA, USA
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16.40- 17.05
New and innovative data analysis methods in quantitative real-time RT-PCR
Speaker:
Michael Pfaffl, Ph.D., Reader in Physiology, Technical University of Munich
and Editor of Gene-Quantification Info., Germany
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17.05 - 17.30
SPUD and 5'/3' assay for QC
Speaker:
Tania Nolan, Ph.D., Sigma-Genosys, UK
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17.30 - 17.55
High Resolution Melt
Speaker:
Charles Hardwick, Ph.D., Field Applications Consultant, Roche Applied Science,
AL, USA
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17.55 - 18.20
Genotyping with Confidence: Fortified Probes Achieve Powerful Discrimination
Speaker:
Ben Sowers, Ph.D., Research Associate, Biosearch Technologies Inc., CA, USA
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18.45 - 20.30
Networking and Reception
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Note: Program and speakers may change
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Tuesday, 30th, October 07
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8.00 - 9.00
Breakfast and networking
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9.00 - 9.10
2nd day opening remarks
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9.10 - 9.35
Quantitative mRNA analysis in single human embryonic stem cells
Speaker:
Dr. Anders Ståhlberg, Stem Cell Center, Lund University, Sweden
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9.35 - 10.00
Large Scale Cell-to-cell Variations in Gene Expression
Speaker:
Sanjay Tyagi, Ph.D., Public Health Research Institute, NJ, USA
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10.00 -10.25
Single ES cell profiling reveals unique microRNA expression signatures and heterogeneities
Speaker:
Caifu Chen, Ph.D., Director, Assays/Arrays R&D, Applied Biosystems, CA, USA
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10.25 - 10.45
Coffee Break |
10.45 - 11.05
Microfluidic Pathogen Detection System
Speaker:
Allen Northrup, Ph.D., President, CEO, MicroFluidic Systems, Inc., CA, USA
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11.05 - 11.30
Massively parallel real-time PCR and its applications
Speaker:
Colin Brenan, Ph.D., Co-Founder, CTO, BioTrove Inc., MA, USA
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11.30 - 11.55
Towards High-Throughput Single-Cell qRT-PCR
Speaker:
Luigi Warrren, Ph.D., Stanford University, CA, USA
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11.55 - 12.20
High Throughput microRNA Profiling from Single Cells Using Real-Time PCR and Dynamic
Arrays
Speaker:
Carl L. Hansen, Ph.D., University of British Columbia, Michael Smith Laboratories,
Vancouver BC
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12.20 - 13.10
Lunch Break |
13.10 - 13.35
Significant changes in microRNA expression profiles during human stem cell differentiation
Speaker:
Christopher Adams, Ph.D., R&D Manager - Epigenetic, Invitrogen Corporation,
CA, USA
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13.35 - 14.00
A Validation Method for siRNA Hits by Real-Time qPCR Suitable for Automation
Speaker:
Gregory L. Shipley, Ph.D., Asst. Professor, Director, Quantitative Genomics
Core Laboratory,University of Texas Health Science Center- Houston, USA
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14.00 - 14.25
Towards mammalian de novo genome sequencing in a single day
Speaker:
Ronaghi Mostafa, Ph.D., Principal Investigator, Stanford University, CA,
USA
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14.25 - 14.50
Molecular Regulation of the Tracheobronchial Stem Cell Hierarchy.
Speaker:
Susan D. Reynolds, Ph.D., Associate Professor, Department of Pediatrics,
National Jewish Medical Research Center, CO, USA
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14.50 - 15.50
Poster Session and Coffee Break
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15.50 - 16.15
A Real Time qPCR Assay for the Quantitation of Genetically Diverse HIV-1 Specimens
Speaker:
Klara Abravaya, Ph.D., R&D Director
Associate Research Fellow, Abbott Molecular Inc., IL, USA
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16.15- 16.40
Rapid Pathogen Identification, Strain Typing, and Drug
Resistance Profiling using PCR/ESI-MS
Speaker:
Rangarajan Sampath, Ph.D., Executive Director, IBIS Biosciences, CA, USA
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16.40 - 17.05
The need and requirements for improved infectious disease diagnostic technologies
in the developing world
Speaker:
Mickey S. Urdea, Ph.D., Managing Partner, Halteres Associates, LLC., CA,
USA
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17.05 - 17.10
Closing Remarks
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Note: Program and speakers may change
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Wednesday, 31st Oct.- Thursday, 01st Nov., 07
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09.00-17.00: Hands on qPCR Course
Lead Instructor:
Robert Sjoback, Ph. D., TATAA Biocenter
This two day hands-on qPCR course offers researchers, students and professionals
an explanation, teaches how to use and interpret real-time PCR gene expression data
and classify samples based on real-time PCR expression profiling. The course offers
a unique opportunity to the participants to work on different instruments available
in the field for quantification.
Details
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09.00-17.00: Biostatistics and Gene Expression Profile Course
Instructors:
Mikael Kubista, Ph. D., Founder, TATAA Biocenter
Amin Forootan, President, MultiD Analyses AB, Sweeden.
This two day course on biostatistics application in qPCR offers researchers an explanation
on statistics applicable to qPCR and teaches how to use statistics to interpret
real-time PCR gene expression data and classify samples based on real-time PCR expression
profiling.
Details
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